The principle and method of polyacrylamide gel electrophoresis (SDS-PAGE).The following sections describe the procedure starting from electrotransfer of proteins to the membrane.įollow the links below for the methods used to generate antibodies, and the principle and method of SDS-PAGE. The bound enzyme activity is used to detect the target protein and visualized by a chemiluminescent or chromogenic method. The membrane with transferred proteins is then probed with a primary antibody (an antibody specific for the target protein), washed, and reacted with a secondary antibody labeled with an enzyme, such as horseradish peroxidase (HRP). In Western blotting (WB), target proteins are transferred to a hydrophobic membrane after SDS-PAGE and detected using specific antibodies.Īfter SDS-PAGE, a membrane is placed on the gel, to which the separated proteins in the gel are electrophoretically transferred. FAQs – In Vivo Isotype Control AntibodiesĪdvanced MHC Tetramers and Antibodies Searchįind products for Western blot The principle.Protein-Protein Interaction Detection Technology.FGF-Max (Recombinant human FGF-1/FGF-2 chimera).Afamin/Wnt3a CM and recombinant protein Show submenu.Non-Classical MHC Tetramers Show submenu.MHC Class I & Class II Tetramers Show submenu.SARS-CoV-2 MHC-Peptide Screening Kit Bundles.QuickSwitch™ Custom Tetramer Kits+ Show submenu.Custom MHC Tetramers and Monomers Order Form.The BFGs didn't complain when I hit the brakes with everything I had, and I came to a quick, uneventful stop. The final portion of the wet autocross called for a full ABS stop. On my second run, I felt confident enough to give it a little more gas through the turn. This turn caught me by surprise, but luckily, I was able to correct my line and make it through without incident. Next was a sweeping left-hander, which narrowed toward the end for a slightly decreasing radius corner. Going into the slalom, you could tell the ground was plenty wet, but the car's steering remained responsive, allowing me to finish the course without plowing through too many cones. Launching the DSG-equipped GTI (kept in sport mode for the duration of the test), I experienced a bit of wheelspin on takeoff, but was able to gain traction not long after. We first drove the course on the BFGs, which held up reasonably well against the slippery asphalt surface. The GTI was the weapon of choice for this demonstration, and the tires compared against the Comp-2 were the Kumho Ecsta SPT and Hankook Ventus V12 Evo. Our first test took us to the parking lot of the Auto Club Speedway, where BFG set up a wet autocross course. The Comp-2 will take the g-Force Sport's place in the lineup as the brand's Ultra High-Performance (UHP) offering, slotting beneath the top dog g-Force T/A KDW summer tire in price. The Comp-2 carries a UTQG (Uniform Tire Quality Grading) tread wear rating of 340/AA/A, identical to the current g-Force Sport's rating. The Comp-2's directional tread pattern is similar to the g-Force Sport it replaces, with BFG's signature "g-Hooks" and shallow shoulder blocks. In addition, the Comp-2's structure is reportedly 40 percent more rigid, with the addition of g-Control wedge inserts in the sidewall, and BFG's Equal Tension Containment (ETEC) system surrounding the tire's internals. The Comp-2 moniker stands for compound two, a next-gen silica-infused tread compound that BFG says helps the new tire achieve 30 percent better wet grip and 8 percent better dry grip than the outgoing g-Force Sport. BFGoodrich picked California's Auto Club Speedway as the test site for the launch of its g-Force Sport Comp-2, the successor to the current g-Force Sport.
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